Techniques in Neuroscience and Cell Biology.

Catalog description

Semester course; 2 lecture and 2 laboratory hours. 3 credits. Students are encouraged to take NEUS 609 , or equivalent, prior to enrolling in the course. Designed to provide in-depth coverage of techniques commonly used in neuroscience and cell biology. Topics include tissue processing for light and electron microscopy, immunocytochemistry, laser confocal microscopy, protein purification and analysis, molecular approaches to the study of the nervous system, genetic manipulation of protein expression, gene arrays, transgenic and knockout animal modes, and electrophysiological techniques including single and multi-unit extracellular recording, sharp intracellular recording and patch clamp recording. Graded as pass/fail.