Advanced Research Methods in Biotech

Catalog description

Methodology and application in biotechnology. This course is primarily lab based and will prepare students for independent research project. The following topics will be covered: genetic engineering techniques for introducing a gene for a desired protein into a cell with vectors, techniques for growing bacteria and yeast to produce a desired protein, cell transformation by electroporation, blue/white screening for selection of a colony with successful transformation with the desired vector, identification of a plasmid with PCR and DNA sequencing, use of enzyme assays to measure protein concentration in solutions, fed-batch yeast fermentation, mammalian cell culture techniques, and analysis of protein expression by Western blot. Lab fee.