Adv. Research Methods in Biotechnology

Catalog description

Methodology and application in biotechnology. The following topics will be covered: genetic engineering techniques for introducing a gene for a desired protein into a cell with vectors, techniques for growing bacteria and yeast to produce a desired protein, cell transformation by electroporation, blue/white screening for selection of a colony with successful transformation with the desired vector, identification of a plasmid with PCR and DNA sequencing, use of enzyme assays to measure protein concentration in solutions, fed-batch yeast fermentation, mammalian cell culture techniques, and analysis of protein expression by Western blot. Lab fee.