Advanced Molecular Techniques

Catalog description

A continuation of the HS 3102 Molecular Techniques Lab course where basic techniques introduced in the previous course are reinforced and built upon through an objective based approach schema in which students presented with a scenario simulating either the clinical or research molecular lab are required to process samples from receipt to report. At the bench, students learn the effective organizational and technical skills for processing multiple samples for gDNA extraction from a variety of specimen types, DNA quantitation, PCR amplification, gel electrophoresis and proper visualization and documentation of results. There is a greater focus on carrying out experimental objectives in accordance with proper quality assurance and quality control guidelines while placing a stronger emphasis on delivering timely, accurate and reproducible results. Proper documentation habits are adhered throughout the experimental process from sample receipt to final analysis and reporting of experimental results. PCR troubleshooting basics, restriction digestion and restriction mapping, as well as an introduction to molecular cloning. Effectively, the student solidifies a very strong foundation in all the basic techniques of the molecular lab with an introduction to those further applications that will be reinforced and built upon in the Senior Year. Course Fee: $38.11