Imaging: Biological Light Microscopy

Catalog description

This intensive laboratory and discussion course will provide participants with the theoretical and practical hands-on knowledge to utilize emerging imaging technologies based on light microscopy. The lecture portion (3 units) is open to 24 students, while the lab portion requires a separate registration and has a maximum enrollment of 12 students (2 Units). In order to take the lab portion you need to sign up for the lecture portion as well. Topics of the lecture portion include microscope optics, resolution limits, fluorescence, confocal microscopy, image analysis, two-photon, TIRF, FRET, photobleaching, super-resolution (SIM, STED, STORM/PALM), light sheet microscopy, and live-cell imaging. We will be using a flipped class format in which students will watch iBiology video lectures before class, and class time will be used for extensive discussion of assigned questions. The lab portion involves extensive in-class use of microscopes in the CSIF and NMS microscopy facilities under guidance of the facility directors and staff. Applications include using fluorescent probes to analyze dynamics of subcellular protein localization in live cells and neuronal signaling in the brain of live zebrafish. Students will be able to choose an advanced imaging technique for their final project and even bring their own samples.