Advanced Imaging

Catalog description

Students will gain an understanding of (1) the physical design of the compound light microscope and its relationship to image formation; (2) the physics of image formation; and (3) selected biological microscopic imaging techniques. Students will gain mastery of (1) the hands-on use of the compound light microscope, (2) basic sample preparation, (3) quantitative image analysis, and (4) the production of publication-quality images. Students will gain experience in the use of specific biological imaging techniques including wide-field fluorescence and confocal imaging, time-lapse imaging, and three-dimensional volume reconstruction. Three-hour lecture/lab.