Molecular Diagnostics I

Catalog description

Pre-requisites: CILC 6046. Through distance learning modality this course presents the basic principles of molecular diagnostics used in clinical and research laboratories. The student is exposed to advances in molecular diagnostics in industry and in research procedures. Topics to be discussed are: purification and quantification of DN A and RNA, gel electrophoresis, visible spectrophotometry of nucleic acids, restriction enzyme analysis of DNA, DNA amplification and optimization of Polymerase Chain Reaction (PCR) and real time PCR. The course will also present the topics of microarrays, quality control, assurance, and assay validations for molecular diagnostics and General Laboratory Practices (GLP´s) for the molecular diagnostics. Applications of these techniques in the molecular di agnosis of prevalent diseases will be discussed. Topics will be presented through: Videoconferences, synchronous and asynchronous group discussions, video animations, discussion forums, interpretation of scientific papers and presentations, among others.