Investigations in Molecular Genetics II

Catalog description

This course will involve training in how to design, conduct, and analyze experiments in molecular genetics. In this course, students will participate in the generation of mutant cells by site specific recombination of mutant constructs introduced into diploid yeast cells. The mutant gene constructs will be generated by the students in the laboratory by the deletion and/or insertion of DNA or DNA markers respectively into a selected target gene clone. Traditional transformation and electroporation techniques will be used for generation of mutants. Candidate mutant cells will be analyzed by diagnostic Southern Blot and PCR analysis of genomic DNA. Techniques for genetic analysis of gene function through analysis of haploid cells will be applied. The results of these experiments will generate novel mutants that will reveal information on gene structure-function relationships.