Investigation in Molecular Genetics I

Catalog description

This course will involve training in how to design, conduct, and analyze experiments in molecular genetics. For this course, students will learn and acquire hands on experience in bacterial culture and the use of prokaryotic and eukaryotic cloning vectors. Cloning from a genomic or CDNA library, and physical characterization of selected DNA will be performed using procedures such as restriction mapping and DNA sequencing. Computer aided analysis of DNA and amino acid sequences will be used. These studies wi ll provide information leading to the identification of gene sequences from a variety of organisms. These studies will be expected to answer new questions and to generate novel data, as opposed to reproducing experiments that have been performed before.