Investigations in Filamentous Proteins II

Catalog description

This course will involve training in how to design, conduct, and analyze independent research in the Structure and Function of Filamentous Proteins. Mutated filamentous protein gene(s) will be used for generating chromosomal mutants. This course will inclu de studies in the functional analysis of filamentous proteins mutants through assays for secretory function, distribution of cell polysaccharides and cytoskeletal proteins, protein phosphorylation of the mutant protein, and effects on cell division. The co urse will incorporate the techniques of Fluorescence Microscopy, for analysis of immunochemical and other protein specific dyes, and immunoprecipitation of radiolabelled filamentous proteins. These experiments will generate novel information on the functio n of filamentous proteins in non -muscle cell systems. These studies will be expected to answer new questions and to generate novel data, as opposed to reproducing experiments that have been performed before.