Investigations in Filamentous Proteins I

Catalog description

This course will involve training in how to design, conduct, and analyze independent research in the Structure and Function of Filamentous Proteins. This course will include studies in identification, isolation, and mutagenesis of selected DNA regions enco ding a portion of a filamentous protein. These selected DNA fragments will be obtained by PCR techniques from a cloned DNA template. Mutations generated will be confirmed by direct DNA sequencing of the mutated DNA fragment. The resulting experiments will inquire into the role of specific amino acids in the function and control of filamentous proteins. These studies will be expected to answer new questions and to generate novel data, as opposed to reproducing experiments that have been performed before.