Biochemistry of Proteins

Catalog description

The structure and function of various proteins (i.e. antibodies, enzymes, nucleic acid, binding proteins, cytoskeletal proteins, and membrane associated proteins) will be examined in detail. Protein folding, denaturation, and refolding will be reviewed. Ap plications of site directed mutagenesis, nuclear magnetic resonance, X-Ray crystallography, and molecular modeling will be covered. Enzyme kinetics will be examined in detail with emphasis on the analyses of catalytic mechanisms, subunit interactions, allo steric effectors, and inhibitors. sieve, affinity, and high pressure liquid column chromatography, Western Blotting, mass spectrometry, Edmon degradation, composition analyses, S.D.S. gel electrophoresis, isoelectric focusing, and PKA determinations, and other procedures that are used in the purification and analysis of proteins will be considered.