Elementary Biochemistry Laboratory

Catalog description

Selected experiments to illustrate major biochemical principles and techniques. B M B 212 B M B 212 Elementary Biochemistry Laboratory (1)B M B 212 exposes students to techniques typically used in industrial and academic laboratories to isolate proteins, perform enzyme kinetics, characterize carbohydrates and lipids, and study molecular biology. Data interpretation and conceptual understanding are emphasized.Specifically, students determine a weak acid's buffer range with a pH meter; quantify protein concentrations using a spectrophotometer; partially purify acid phosphatase from wheat germ, using ammonium sulfate precipitation, centrifugation and dialysis; characterize acid phosphatase kinetics; subject glycogen to acid and salivary amylase hydrolysis, then compare products using thin layer chromatography; isolate plasmid DNA from E.coli, then digest the plasmid DNA with restriction enzymes and analyze the products using agarose gel electrophoresis; make soap from commercial oils and fats; and lastly, identify fatty acids using a gas chromatograph.Students write laboratory reports to present their findings and correlate theory with actual experimental results. Written quizzes assess conceptual understanding of experiments. Teaching assistant evaluations judge the student's level of laboratory skill achievement, preparation, and ability to work with others in a professional manner.A solid chemistry knowledge base (CHEM 012 and either CHEM 034 or 038), previous laboratory experience, and the ability to work with mathematical word problems are expected of all students enrolled in the course. Biochemistry focuses on the chemistry of living organisms. This course provides basic biochemistry laboratory skills and exposure to widely-used methodology to develop a fundamental understanding of biochemistry needed for advanced courses in the student's major and compatible with the student's career interests. Outside resources for the student include reserved books and a course web site: www.bmb.psu.edu/courses/daniel/BMB212/default.htm.