Quantitative Imaging and Analysis for Biologists (QIAB)

Catalog description

This course will provide an introduction to the fundamentals of modern light microscopy and image analysis using the free software package, Fiji, as a guide. Topics include fundamentals of basic and advanced light microscopy, image data optimization, and commonly-used processing and analysis tools such as filters, segmentation, tracking, and simple macro programming. The goal is to provide students with the background and confidence required to pursue more advanced quantitative imaging methods as the need arises in their research. Students will be graded based on their active participation in class, completion of hands-on Fiji exercises, and a final presentation describing an application of at least one of the analysis methods covered in class to their own image data. CAMB 7090 is limited to 2nd - 4th-year graduate students who have previous/current experience with light microscopy. Permission to enroll from course directors is required for all students.