Introduction to Plant Molecular Biology

Catalog description

Hands-on introduction to modern molecular biology techniques. Isolation of SNA from tobacco leaves, isolating a plant gene through polymerase chain reaction (PCR), cloning DNA fragments in plasmid vectors, bacterial transformation and plasmid DNA purification, restriction digestion and gel electrophoresis, gene transfer and expression of reporter genes in plant cell lines through a biolistic gene gun. Field trips, poster assignment and poster presentation are mandatory. This course is part of the Summer College in Biotechnology and Life Sciences (SCIBLS) and other pre-college, transitional and early-college programs. Students must have no more than 30 credit hours. Deparment approval required.