Cryoelectron Microscopy of Macromolecular Assembles

Catalog description

This comprehensive course covers the theory and practice of solving molecular structures by electron microscopy. The course starts with optics, sample preparation, and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: electron crystallography, single particle analysis, and electron tomography. The course ends with a discussion of map interpretation and molecular fitting. This is predominantly a lecture course involving one 2-hour lecture per week accompanied by a discussion session and an occasional practical session using the facilities at the New York Structural Biology Center. Lectures are given by expert electron microscopists from around New York City, and students from various campuses are encouraged to attend.