Cell-Based Assays. 2 credits

Catalog description

Focus on 1) basics of eukaryotic cell culture; 2) various cell based assay techniques; 3) Real-Time PCR based functional analysis of the signaling pathways. Students will maintain their cell cultures for the duration of the experiments, perform at least one functional assay and analyze the resultant data. Students are expected to learn the properties and limitations of each cell based assay and should be able to explain their results regardless of the outcome. Each student will be responsible for submitting a written report summarizing the design of their experiments and its results. Each report will include the following sections: Introduction, Methods, Results and Discussion, and a special Troubleshooting section. Notes: A lab fee of $300 will be charged per student for lab supplies. Offered by School of Systems Biology . May not be repeated for credit.