Molecular Methods in Biology

Catalog description

This course provides an introduction to the theoretical and practical basis for understanding key experimental techniques used in modern molecular biology research. The students receive practical hands-on experience in selected techniques. In parallel, the lectures provide molecular principles and mechanisms underlying the experimental techniques. The experimental techniques include basic recombinant DNA techniques, real time polymerase chain reaction (RT-PCR), microarray, protein analysis, recombinant protein production and purification, protein-protein interaction, protein localization, and gene transfer in eukaryotic systems. In addition, hands-on training in genomics and proteomics is provided through the use of modern bioinformatics software. The students are expected to gain critical skills to design relevant molecular experiments to address biological questions and to evaluate experimental data and conclusions.