Biochemical Energetics Laboratory

Catalog description

Students will perform experiments that will investigate the energetics of biomolecules, bimolecular structural changes and bimolecular reactions. Experiments utilized will investigate solution partition, solvation, enzyme kinetics, enzyme inhibition, energetics of protein unfolding and ligand binding. Examples of techniques to be used include absorption and fluorescence spectroscopy, centrifugation, electrophoresis, blotting, and equilibrium dialysis. Emphasis will be placed on standard laboratory calculations, solution preparation, experimental design and record keeping. Critical evaluation and statistical analysis of data are stressed.