Current Proteomics and Bioinformatics

Catalog description

This course is designed for graduate students across the university who wish to acquire a better understanding of fundamental concepts of proteomics and related bioinformatics as well as hands-on experience with techniques used in current proteomics. Lectures will cover protein/peptide separation techniques, protein mass spectrometry, and biological applications which include quantitative proteomics, protein modification proteomics, interaction proteomics, structural genomics and structural proteomics. Also, it will cover experimental design, basic statistical concept and issues related to high-dimensional data from high-throughput technologies. Laboratory portion will involve practice on the separation of proteins by two-dimensional gel electrophoresis, molecular weight measurement of proteins by mass spectrometry, peptide structural characterization by tandem mass spectrometry. It will also include bioinformatics tools for protein identification and protein-protein interaction networks. The instructors' research topics will also be discussed. Recommended preparation: CBIO 453, CBIO 455, and PQHS 431 .