Protein Engineering

Catalog description

This course will provide an in-depth examination of protein engineering topics and their applications. In particular, this class will cover the design and expression of recombinant proteins, purification strategies, and the incorporation of non-natural amino acids using a bacterial system. Specifically, amino acid sequences that dictate well-defined secondary structures such as beta-sheets, alpha-helices, and leucine zippers will be studied. Tissue engineering examples from the literature such as incorporation of bioactive sequences to promote specific cell response (e.g., cell adhesion sites and protease degradation sequences). In addition, this course will explore the application of protein engineering in drug delivery, electrochemical technology, sensors, and nanoparticle assembly. Current computational techniques for protein design and directed evolution methods will also be explored. Offered as ECHE 386 and ECHE 486 .