Biotechnology Laboratory: Eukaryotic Molecular and Cellular Biology

Catalog description

This spring course provides hands-on laboratory experience in mammalian cell culture and molecular and cell biology. Specific topics include: General laboratory safety, good laboratory practices (GLP), standard operating procedures (SOPs), buffers, media, and other reagent preparation, sterile technique, manipulation of mammalian cells, mammalian cell culture, work with DNA and RNA, polymerase chain reaction (PCR) techniques including quantitative reverse transcription (RT-qPCR) and molecular cloning, reporter assays, transfection, immunoprecipitation, immunofluorescence, and protein gel electrophoresis and blotting. This course, together with BIOC 502A and 502C, comprise a one-semester lab course that provides students with a comprehensive introduction to skills used in modern biotechnology laboratories. Students may take one, two, or three of these courses in a single semester. Suitable for biochemistry MS students interested in biotechnological and/or industry careers. All other graduate students and/or undergraduate students must contact the instructor for permission to enroll. Prereq: BIOC 500 and BIOC 501 or Requisites Not Met permission.