Cell Biotechnology: Cell Culture, Immunocytochemistry and Bioimaging

Catalog description

Fundamentals of creating and maintaining cell culture for the purpose of experimental design and analysis. Cell culture technique, cell behavior, cell signaling and regulation, histology, transgenic fluorescent protein expression, immunocytochemistry and various bioimaging techniques including live cell, epi-fluorescent and confocal microscopy. Working with established cancer-derived cell lines and primary neural disassociated cultures, students perform various experimental techniques for cell imaging preparation and acquisition. Focuses on image analysis and presentation using ImageJ. During the second half of the semester, students propose and develop an experimental project in which they will collect data, do analysis and prepare the results for a final project presentation.