Advanced Separation Science - HPLC

Catalog description

This course aims at providing students with fundamental skills and knowledge in advanced separation science, in particular HPLC. The course will enable students to understand, develop and execute analytical protocols involving recent HPLC methodologies and instrumentation. The lecture will consider all common techniques in liquid chromatography such as gradients, normal and reversed phase, gel permeation, ion exchange, bioaffinity, and chiral columns, as well as RI, UV-vis, fluorescent, luminescent, electrochemical, and MS detection. Students will learn by lectures, class activities, and homework assignments how to plan analytical tasks considering the available HPLC techniques in a modern routine laboratory, as well as how to optimize the conditions in order to obtain sufficient analytical performance parameters in terms of selectivity, detection limit, cost, and analysis time. The lab will introduce the students in reverse phase HPLC using RI and UV-vis detection. Practical examples in the lab section will include food and soil analysis considering analytes and separation problems that can only be addressed by HPLC. Two lecture and one lab meetings per week. Prerequisite(s): A CHM 225 or A CHM 250. May not be offered in 2024-2025.